Part:BBa_K4831003

MerB alkylmercury lyase from P. aeruginosa (seq. harmonized to Synechocystis sp. PCC6803)

Overview

MerB protein is an Organomercurial lyase that cleaves the bond between C-Hg and releases Hg(II) in addition to the appropriate organic compound. It can be used as a combination with merA, which is a mercuric reductase. Together or alone, merB can be used to increase the resistance to organic mercury (methyl mercury) or mercuric compounds like mercuric (II)chloride. It can be used as a tool for bioremediation. This part is designed to be used in Synechocystis sp PCC6803.

Design Notes

The sequence is modified with restriction sites for position 5 in a 7-fragment Type IIs assembly with Bbs1. The start codon ATG is also removed. The sequences codons have been harmonised for optimal translation in Synechocystis sp. PCC 6803 (changed codons: 164, GC-change 56.6 to 51.3%). The part has been checked for illegal restriction sites (Bsa1 and Sap1).

NOTE! The starting codon ATG has been removed to be compatible with our composite part (reading frame shift).

Source

Pseudomonas aeruginosa strain ARY1 organomercurial lyase (merB) gene, complete cds. https://www.ncbi.nlm.nih.gov/nuccore/FJ613641.1

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal EcoRI site found at 589
  Illegal PstI site found at 143
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal EcoRI site found at 589
  Illegal PstI site found at 143
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal EcoRI site found at 589
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal EcoRI site found at 589
  Illegal PstI site found at 143
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal EcoRI site found at 589
  Illegal PstI site found at 143
  Illegal NgoMIV site found at 412
  
• 1000
  COMPATIBLE WITH RFC[1000]